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KMID : 0360220150560040586
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Journal of the Korean Ophthalmological Society
2015 Volume.56 No. 4 p.586 ~ p.591
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Effect of Bimatoprost on the Permeability of Trabecular Meshwork Cell Monolayer
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Chang Bi
Kim Jae-Woo
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Abstract
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Purpose: To investigate the effects of bimatoprost on the permeability of cultured human trabecular meshwork cells (HTMC) monolayer.
Methods: HTMCs were cultured until confluency in the inner Transwell chamber and then exposed to benzalkonium chloride, brimonidine, latanoprost or bimatoprost for 1 week. Carboxyfluorescein permeability through the HTMC monolayer was measured using a spectrofluorometer after 2 hours in the outer chamber. Cellular viability was assessed using the MTT assay.
Results: Each drug diluted at 1/1000X did not affect the cellular survival (p > 0.05). Brimonidine, latanoprost and bimatoprost did not affect the carboxyfluorescein permeability through the HTMC monolayer (p > 0.05). The carboxyfluorescein permeability was not different between latanoptost and bimatoprost after 1 week of exposure (p > 0.05).
Conclusions: Bimatoprost, a drug known to increase trabecular outflow, does not affect the carboxyfluorescein permeability through the HTMC monolayer. Thus, the effect on the trabecular outflow of bimatoprost may not be significant.
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KEYWORD
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Bimatoprost, Carboxyfluorescein, Permeability, Trabecular meshwork cells
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